PCR laboratory
Laboratory equipment
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laminar flow chambers
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eppendorf gradient function thermocyclers -classical PCR
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temoblocks with shaking function
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laboratory centrifuges
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epMotion pipetting station
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Roche thermocycler - Real-Time PCR
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mini-centrifuge
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gel documentation system
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BioSpectrometer
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horizontal electrophoresis kit
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vortex
Methodology used and research conducted
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isolation of DNA and RNA directly from clinical specimens as well as from proliferated viral and bacterial pathogens
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identification of pathogens in material of veterinary origin
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determination of genes related to antibiotic resistance and virulence of selected bacteria
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quantitative studies of selected bacterial and viral pathogens
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gene expression analysis
Laboratory of bacteriology and mycology
Laboratory equipment
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densitometers
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light microscope with camera
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bacterial colony counter
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laboratory shaker
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lamps with magnifying glasses
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laboratory balance
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incubators
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incubator with cooling function
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dispensers for antibiogram
Methodology used and research conducted
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isolation and identification of selected bacterial and mycological pathogens from various materials of veterinary origin
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performance of antibiograms and antimycograms by the disk-diffusion method and MIC method (e-tests, microdilution method)
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quantitative testing for selected pathogens (10-fold dilutions)
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determination of biofilm production capacity by selected veterinary pathogens
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storage of isolated strains
Virology laboratory
Laboratory equipment
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laminar flow chamber
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incubator with CO2 flow
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contrast-phase inverted microscope
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fluorescence inverted microscope
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water bath with cooling and shaking option
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egg incubator
Methodology used and research conducted
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multiplication and titration of animal viruses in cell cultures and chicken embryos
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evaluation of the effects of antiviral substances on the multiplication of viruses in cell cultures, with a preliminary determination of the stage of the viral cycle they affect
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evaluation of the virucidal effect of chemical disinfectants and antiseptics used in the veterinary field
Laboratory of immunology and cell culture
Laboratory equipment
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laminar flow chamber
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incubator with CO2 flow
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automatic cell counter
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centrifuges with cooling option
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microplate reader
Methodology used and research conducted
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acquisition of primary cell cultures (chicken embryo fibroblasts, fish and mammalian peripheral blood leukocytes, fish and mammalian organ lymphocytes and phagocytes)
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functional evaluation of cells of the immune system (bacterial engulfment capacity and aerobic burst of phagocytes; proliferative activity of lymphocytes stimulated by mitogens and specific antigens; cytokine production) by colorimetric methods and using flow cytometry
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immunophenotyping of cells using a flow cytometer
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working with cell lines from the ATCC collection
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evaluation of cytotoxicity of substances using colorimetric cytotoxicity assays (MTT, NRU, LDH, SRB)
Diagnostic laboratory
The laboratory offers services in the following areas:
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isolation and identification of selected veterinary pathogens from clinical specimens (classical bacteriology and mycology, identification using PCR technique)
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determination of the antibiotic resistance profile of isolated pathogens using the disk-diffusion method (antibiogram, antimycogram)
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preparation of auto vaccines (selected pathogens)
Equipment remaining for use by all laboratories and the diagnostic laboratory:
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autoclaves
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ultrasonic sterilizer
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laboratory dryer
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distiller
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ultra-low freezers
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liquid nitrogen tanks
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laboratory pH meter
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water/ice bath
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magnetic stirrer
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laboratory scale
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vacuum pump
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ice maker
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dishwasher
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centrifuges
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refrigerators
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uv lamps
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vortex
