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Department of Pharmacology and Toxicology

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Workrooms

LABORATORY OF LIQUID CHROMATOGRAPHY AND PHARMACOKINETIC ANALYZES

& LABORATORY OF BIOLOGICAL SAMPLE PREPARATION

Laboratory equipment

  • ultrapressure liquid chromatograph (Waters ACQUITY UPLC I-Class PLUS) coupled with a tandem mass spectrometer (Waters Xevo TQ-XS Triple Quadrupole Mass Spectrometry) and an ultraviolet detector,
  • ultrapressure liquid chromatograph (Waters ACQUITY UPLC I-Class PLUS) coupled with a fluorescence and diode detector,
  • ultrapressure liquid chromatograph designed to work with micro flows (Waters ACQUITY UPLC M-Class) and to work with a unique ion source ionKey/MS (Waters),
  • UniSpray ion source (Waters UniSpray Ion Source) for the mass spectrometer (Waters Xevo TQ-XS), significantly increasing the sensitivity of the MS,
  • high pressure liquid chromatograph (Agilent 1100 Series) with diode detection,
  • high pressure liquid chromatograph (Agilent 1100 Series) with fluorescence detection,
  • other devices: scales, pH meters, shakers, solid phase extraction manifold, TurboVap liquid evaporator, refrigerators, freezers, etc.

 

Methods and research & The offer of the Laboratory

  • development and validation of methods for the quantitative analysis of small molecule chemotherapeutics (mass below 2000 Da) using high-performance liquid chromatography coupled with light detection and/or tandem mass spectrometry,
  • preparation and isolation of low molecular weight chemotherapeutics (mass below 2000 Da) from biological and non-biological material,
  • quantitative analysis of small molecule chemotherapeutics (mass below 2000 Da) in biological and non-biological material,
  • pharmacokinetic studies of drugs used in veterinary medicine, including tetracyclines, quinolones, NSAIDs, antiparasitic drugs,
  • pharmacokinetic interactions at the absorption level,
  • determining the adsorption of drugs on filters used in renal replacement therapy in humans,
  • pharmacokinetic studies of antimicrobial chemotherapeutics used in humans.
 

 

LABORATORY OF IMMUNOPHARMACOLOGY

& BIOTECHNOLOGY

Laboratory equipment

  • flow Cytometer (BD FACSCelesta)
  • Whole Body Plethysmography (WBP) o measure respiratory parameters in conscious unrestrained mice with he possibility of nebulization (Buxco FinePoint Whole Body Plethysmography),
  • tissue homogenizer (Tissuelyser II)
  • automated cell harvester (MicroBeta FilterMate Cell Harvester),
  • automated Cell Counter (EVE)
  • autoclave (Laboklav 25),
  • incubators (Memmert, SL Shel LAB, Stuart),
  • laminar flow biohazard cabinets biohazard (Kojair, ESCO),
  • inverted optical microscope equipped with camera (Olympus CKX41),
  • inverted fluorescence microscope (Olympus IX71),
  • laboratory centrifuges (Allegra X-15R, Allegra 64R, Avanti I-15R),
  • microplate readers (Elx 800),
  • other devices: laboratory balances, pH meters, laboratory shakers, laboratory cradle, refrigerators, freezers etc.

 

Methods and research & The offer of the Laboratory

  • isolation of: (a) lymphocytes from lymphoid and non-lymphoid organs; (b) luteal and follicular cells,
  • cell cultures (lymphocytes from peripheral blood and lymphoid and non-lymphoid organs),
  • immunophenotyping of immunocompetent cells by flow cytometry,
  • flow cytometric diagnostics of lymphoma and leukaemia in dogs,
  • flow cytometric evaluation of the percentage and absolute count of selected lymphocyte subsets,
  • flow cytometric evaluation of apoptosis of selected lymphocyte subsets,
  • flow cytometric evaluation of proliferation of selected lymphocyte subsets,
  • flow cytometric evaluation of intracellular cytokine production (e.g. IL-10, IFN-γ) by various selected lymphocyte subsets
  • ELISA (Leukotrienes, progesterone, prostaglandin, cytokines etc.) measurement of respiratory parameters in mouse by Whole Body Plethysmograph.

 

LABORATORY OF ATOMIC ABSORPTION SPECTROMETRY

& LABORATORY OF BIOCHEMISTRY

Laboratory equipment

  • AAS spectrometer (PinAcle 900T PerkinElmer),
  • automatic mercury analyzer (AMA 254 HSC),
  • microwave digester (Mars 6),
  • laboratory disinfector (Miele PG8593),
  • UV/VIS spectrophotometer (BECKMAN DU®520),
  • UV spectrophotometer (MARCEL MEDIA BIO PLUS),
  • analytical scales (Radwag XA60/220/X),
  • centrifuge (MPW 351R).

 

Methods and research & The offer of the Laboratory

  • determination of trace amounts of selected elements (mainly metals), e.g. Cd, Pb, As, Cu, Zn, Se, Cr in liquid, solid and gaseous samples and determination of trace amounts of Hg in solid, liquid and gaseous samples without the need for prior mineralization of the sample
  • mineralization of biological material (e.g. animal tissue)
  • determination of the biological effects of pesticides and their interactions both with each other and with other xenobiotics (e.g. metals, drugs),
  • analysis of the influence of pesticides, drugs and metals on the body's antioxidant system,
  • determination of concentration and activity of selected antioxidant elements, e.g.; superoxide dismutase activity, catalase, glutathione reductase, glutathione peroxidase and total oxidative status (TAS) activity,
  • the concentration of vitamin A, C and E and the malondialdehyde (MDA) as a markers of lipid peroxidation.

 

 

LABORATORY OF ECOTOXICOLOGY

Laboratory equipment

  • UV/VIS spectrophotometer (Specord 50 Plus),
  • spectrophotometer (UV-1800 RAYLEIGH),
  • mechanical homogenizer (IKA®T25 digital ULTRA-TURRAX®),
  • basic homogenizer (DI 25),
  • basic rotary evaporator (IKA RV 05),
  • other devices: MEMMERT laboratory dryer, refrigerators, laboratory scales, HYDROLAB HLP 5 water filtration system, IKA® C-MAG HS 7 magnetic stirrer with heating, Vortex IKA® MS 3 basic shaker, MPW 350R centrifuge, water bath with shaker MEMMERT, SANYO MDF-U4186S low-temperature freezer.

 

Methods and research & The offer of the Laboratory

  • research on the impact of various types of xenobiotics (metals, pesticides, drugs) on the animal organism
  • determination of the biological effects of pesticides and their interactions both with each other and with other xenobiotics (e.g. metals, drugs)
  • enzyme tests, e.g. aceticocholinesterase (AChE) activity in the blood and brain as a biomarker of exposure to organophosphate insecticides
  • testing the activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) as markers of hepatotoxic activity
  • determination of the activity and content of selected parameters of the body's antioxidant barrier, eg; superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase activity and total oxidative status (TAS) activity
  • determination of malondialdehyde (MDA) content as a marker of lipid peroxidation
  • extracting poisons from biological material,
  • determination of organic and inorganic substances in the tested material.

 

 

 

LABORATORY OF TISSUE CONTRACTION

Laboratory equipment

  • double set for testing isolated organs Schuler Organbad (Hugo Sachs Elektronik.

 

Methods and research & The offer of the Laboratory

Possibility to assess the impact of:
  • xenobiotics on the contractile activity of the smooth muscle of the gastrointestinal tract and the reproductive system in various species,
  • biologically active substances contained in food on the contractile activity of the smooth muscle of the gastrointestinal tract and the reproductive system in various species

 

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