1. Immunohistochemistry laboratory
The laboratory is equipped with equipment necessary to prepare microscopic preparations (freezing cryostat) and a station for immunohistochemical staining. Staining uses various types of sera containing specific antibodies (primary antibodies) directed against the tested biologically active substances and appropriate secondary antibodies.
The laboratory is able to perform immunohistochemical staining using immunoenzymatic and immunofluorescent methods in which secondary antibodies are labeled with appropriate fluorochromes (a substance capable of fluorescence). To analyze such staining, a microscope equipped with a fluorescence system (an appropriate lamp and a set of filters adjusting the light wavelength) is required. The immunofluorescence method also allows the use of ultramodern analysis and imaging techniques (such as confocal microscopy), which allows for a very detailed analysis of the results obtained.
The immunocytochemistry laboratory also has the ability to perform classic histological stains (e.g. hematoxylin-eosin).
2. Genomics and transcriptomics laboratory
The laboratory is equipped with modern apparatuses which provide efficient platform for conducting researches based on molecular biology techniques.
The main equipment used in our laboratory is listed below:
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Thermocyclers (Eppendorf, Biometra)
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7500 Fast Real-Time PCR System instrument (Applied Biosystems)
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TissueLyser II (Qiagen)
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ZOE cell imager (Bio-Rad)
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RWD Micropipette Puller
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Fluoroimager (Typhoon 9410 GE Healthcare) – nie uzywamy
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a genetic analyzer (ABI PRISM 310 Genetic Analyzer)
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a microinjection system InjectMan® NI 2 FemtoJet®, (Eppendorf)
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a stereomicroscope SteREO Discovery V8 (Zeiss)
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DanioVision (Noldus)
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and number of others, including shakers, thermoshakers, ultra sensitive scales, electrophoresis system, pH indicators.
3. Microscopy laboratory
The laboratory is equipped with fluorescence microscopes (Zeiss) enabling the analysis of preparations made using the immunofluorescence method, and a system for taking digital microphotographs (a high-quality Zeiss digital camera coupled with a computer). The devices also allow for the analysis and imaging of preparations stained using immunoenzymatic and classical methods (light microscopy).
Additionally, the laboratory is equipped with an ultra-modern confocal microscope (Zeiss LSM-700) allowing for a detailed analysis of cellular structures and imaging of the obtained results by scanning individual planes of the microscope preparation. The microscope is equipped with solid-state lasers, which have a huge advantage over constructions using an argon laser. The microscope also allows you to obtain microscopic 3D images of the examined structures.
